Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with [3H]borohydride
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چکیده
منابع مشابه
Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with (3H)borohydride.
A very simple and dirrct radioactive isotope m&hod has been tlwcl~ibed for the quantitative determination of reducing sugars. The method can be adapted to give any degrtte of sensitivity desired. The procedure is of general applicability to the determination of a large class of sugars. The stoichiometry of the reaction makes it a comprehensive procedure which greatly facilitates the analysis of...
متن کاملThe Determination of Reducing Sugars
In the course of an investigation, still in progress, of the physiological activity of a group of soil organisms, it became necessary to determine the quantity of dextrose in a series of solutions. It was desirable to have a method as accurate as the Fehling gravimetric one but which would yield results about as quickly as by direct titration. The reducing sugar present in the 150 cc. of each o...
متن کاملE Analytical Determination of Reducing Sugars
There have been very many methods suggested from time to time for the determination of reducing sugars, and considerable difficulty has been experienced in obtaining one which is sufficiently foolqxoof to give satisfactory results in the hands of all operators. In 1936 von Stieglitz and Home 11) reviewed several methods and recommended the electro* metric determmat~on of the end point with the ...
متن کاملDetermination of reducing sugars with 3-methyl-2-benzothiazolinonehydrazone.
M. (1992) Purification and characterization of human salivarygland prokallikrein from recombinant baculovirus-infected insect cells. Eur. J. Biochem. 206, 225–233. 6. Takada, Y., Skidgel, R. A., and Erdös, E. G. (1985) Purification of human urinary prokallikrein. Biochem. J. 232, 851–858. 7. Noda, Y., Takada, Y., and Erdös, E. G. (1985) Activation of human and rabbit prokallikrein by serine and...
متن کاملThe determination of reducing sugars by titration of ferricyanide.
THE use of ferricyanide for the quantitative estimation of sugar was introduced by Hagedorn and Jensen [1923]. Their method has been extended to other sugars by Hanes [1929] and Hulme and Narain [1931]. These methods involve a preliminary heating for 15 minutes and a subsequent back titration. The fact that ferricyanide is only slightly coloured would seem to make it valueless for a direct titr...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1973
ISSN: 0003-2697
DOI: 10.1016/0003-2697(73)90291-1